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The coding region was cloned into a modified pETM-11 bacterial expression vector which was derived from a pET-24d(+) vector (Novagen) by insertion of a tobacco etch virus (TEV) protease cleavage site following an N terminal hexa-histidine and protein A tag

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10.1164/ajrccm.151.5.7735614 [DOI] [PubMed] [Google Scholar] 103.Grandjean EM, Berthet P, Ruffmann R, Leuenberger P