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High pH fractionation- TMT labeled, combined, and dried samples were resuspended in 300 μL of Buffer A (5% v/v MeCN, 95% v/v H 2 O, 0.1% v/v formic acid) and fractionated by centrifugation using a peptide desalting column (Pierce, #89852)

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Metabolic plasticity allows CSCs to switch between glycolysis, oxidative phosphorylation, and alternative fuel sources such as glutamine and fatty acids, enabling them to survive under diverse environmental conditions